The composition of the rappoport medium. Nutrient media of Rappoport, Endo, Levin, Ressel, Kligler: type of medium, composition, purpose, principle of action. See what "Rapoport Wednesday" is in other dictionaries

Wednesday Rappoport is an enrichment environment and a differential diagnostic environment. In her compound includes: bile broth 10%, 2% glucose, indicator (sour fuchsin, discolored with alkali; colorless in an alkaline medium, red in an acidic medium). Purpose: for the accumulation of typhoid-paratyphoid bacteria during blood culture of the patient, as well as for the approximate differentiation of typhoid-paratyphoid bacteria. Principle d-i: with the growth of typhoid-paratyphoid bacteria due to the fermentation of glucose, diffuse turbidity and reddening of the medium occur. For paratyphoid bacteria, in contrast to typhoid bacteria, the presence of gas in the float.

Wednesday Endo Compound: MPA, lactose, indicator - basic fuchsin, decolorized with sodium sulfite. Purpose: for sieving the test material in order to obtain isolated colonies. Principle d-I based on the ability of some microorganisms to decompose or not decompose lactose (Salmonella does not decompose and form colorless colonies).

Wednesday Levin is weakly selective and differential diagnostic. In her composition MPA, tactose, indicators: eosin and methylene blue, potassium hydrogen phosphate. Appointment and principle of d-I, see the Endo environment.

Ressel Wednesday Compound: MPA, lactose 1%, glucose 0.1%, indicator (water blue and pink to-ta; in an alkaline environment, pink, in an acidic environment, blue). Purpose: for elimination of "suspicious" colonies in order to isolate pure cultures and determine their enzymatic activity in relation to glucose and lactose. Principle d-i:

Wednesday Kligler - combined, differential diagnostic. Compound: MPA, lactose 1%, glucose 0.1%, sodium thiosulfate, iron II sulfate, indicator - phenol red (in an alkaline medium - red, in an acidic medium - yellow). Purpose: for elimination of "suspicious" colonies in order to identify pure cultures and determine their enzymatic activity in relation to glucose, lactose and the formation of H2S. Principle d-i: enterobacteria, which have the enzyme thiosulfate reductase, reduce thiosulfate to sulfite, while hydrogen sulfide is released, which reacts with iron sulfate - as a result, black iron sulfide is formed.

Serological identification of isolated pure culture using adsorbed monoreceptor O- and H-agglutinating salmonella sera. Serological identification is carried out in the agglutination reaction on a stack with agglutinating adsorbed Salmonella O- and H-sera, these sera can be. 2 types: monoreceptor and polyvalent, they are obtained from specific immune sera by the method of adsorption of antibodies according to Castellani.

Serological identification proceeds sequentially in 3 stages:

1) Establishing the belonging of a culture to serogroups A, B, C, D, E, of the genus Salmonella. In this case, polyvalent O-serum containing antibodies against antigenic receptors 2, 3, 4, 7, 9, 10 is used. Monoreceptor O-sera are used against rare serogroups. When diagnosing paratyphoid A and B, typhoid fever, you can use a mixture of O-sera containing antibodies to receptors 2, 4, 9.

2) With a positive result, to determine whether the test culture belongs to a certain serogroup, the agglutination test is set separately with each monoreceptor O-serum that was part of the polyvalent one: receptor 2 belongs to serogroup A, receptor 4 to serogroup B, receptor 7 - to serogroup C, etc.

3) After determining the serogroup, we determine its species affiliation using the agglutination p-tion with adsorbed monoreceptor H-sera against the H-antigens of the 1st phase of Salmonella, which are part of this serogroup. Then, agglutination is carried out with adsorbed H-sera against H-antigens of the 2nd phase and the antigenic formula of the test culture is finally established.

Bacteriocarrier in typhoid fever. What serological reactions can confirm chronic bacteriocarrier? diagnostics used for this purpose. The only evidence of bacteriocarrier is the isolation from the carrier of cultures S. typhi, S. paratyphi A, S. paratyphi B, using auxiliary methods: serological test and allergic skin test with Vi-typhine (it contains Vi-antigen, which, when interacting with Vi-antibodies gives a local allergic reaction in the form of redness and swelling within 20-30 minutes). A positive p-tion with Vi-typhine indicates that there are Vi-antibodies in the org-me and possibly S. typhi.

Chr. bacteriocarrier is confirmed by RNGA. In this case, Vi-diagnosticum is used, because in the formation of typhoid bacteriocarrier, the manifestation of Vi-antibodies is characteristic; diagnostic titer of the district 1:40.

Methods of infection with typhoid fever. source of infection. Infection with typhoid fever occurs only by the oral route. The source of infection is sick people and bacteria carriers (they are dangerous to others for several years after the illness).

Biological products used for the diagnosis, specific prevention and treatment of typhoid and paratyphoid diseases.

1) The typhoid alcohol dry vaccine contains ethyl alcohol-inactivated lyophilized typhoid bacteria, and is used to prevent typhoid fever in adults.

2) Vi-polysaccharide liquid typhoid vaccine - a solution of capsular polysaccharide isolated from a culture of typhoid bacteria, purified by enzymatic and physico-chemical methods. After the introduction of the vaccine, intensive formation of antibodies and the formation of resistance begin in 1-2 weeks. For the prevention of typhoid fever in adults.

3) Alcohol typhoid vaccine enriched with Vi antigen consists of 1 + 2, which are combined immediately before use. The vaccine stimulates cellular immunity and the formation of antibodies to O- and Vi-antigens of the causative agent of typhoid fever. For the prevention of typhoid fever in children 7-14 years old.

4) Bacteriophage typhoid in tablets with acid-resistant coating - tablets from concentrated lyophilized phagolysate of salmonella typhoid, coated with ACP layer or containing pectin. For the prevention of typhoid fever according to epidemiological indications in the family hearth, hospital, locality, etc.

5) Salmonella bacteriophage ABCDE group - a filtrate of phagolysates, the most common Salmonella bacteria (more than 10 species), produced in liquid form and in tablets with an acid-resistant coating. For the prevention and treatment of salmonellosis.

Salmonellosis.

Features of the antigenic structure of Salmonella (O -, H -, Vi-antigens), their chemical nature, localization. Salmonella classification according to Kaufman-White. Serogroups, serovars. O-antigen (somatic) - thermostable, located in the cell wall, by its nature is a polysaccharide-protein-lipid complex. The specificity of this antigen depends on which polysaccharide it is associated with (it is different for Salmonella of different groups). Antigenic receptors are designated by numbers - 1, 2, 3, etc.

K-antigen (case) - thermolabile, located in the cell wall more superficially than O-antigen. Salmonella have M-Ag and Vi-Ag, which are K-antigens.

H-antigen (flagellate) - thermolabile, by its nature it is a protein (flagellin), located in the flagella. In bacteria of the same species, it can consist of two complexes that differ in serological specificity. These are two-phase bacteria, for each of the phases there are corresponding antigenic complexes: antigens of the 1st phase (specific), denoted by the letters a, b, c, etc .; and antigens of the 2nd phase (non-specific) - 1, 2, 3, etc.

Kaufman and White, on the basis of the antigenic structure, divided all representatives of the genus Salmonella into serological groups based on the commonality of O-antigens, where each group has a specific antigen receptor that representatives of other groups do not have. Within the group, Salmonella are divided into species (serovars) according to the difference in the structure of H-antigens of the 1st or 2nd phase.

The main sources of salmonella infection. Pathogens, their morphological properties and biochemical tests used to differentiate Salmonella. PTI is most often caused by Saalmonella, which are members of serogroups B, C, D, E, i.e. obstructions are zooanthroponotic. The source of infection with S. typhimurium (serogroup B) can be. mice, pigeons, poultry and their eggs, secondary m.b. other products are contaminated. The source of S.choleraesuis infection (serogroup C) is pigs; the source of infection with S. enteritidis (serogroup D) is cattle.

Salmonella pathogenicity factors causing food poisoning. They have adhesion and colonization factors, invasion factors. They have endotoxin with a wide range of d-I, many of them have enterotoxins (LT and / or ST-toxins) that disrupt the functions of the adenylate and guanylate cyclase systems of enterocytes --- violation of water-salt metabolism --- diarrhea. Some salmonella have a cytotoxin that disrupts protein synthesis in erythrocytes --- hypersecretion and impaired enterosorption of fluid in the small intestine --- diarrhea.

In pathogenesis, the ingestion of a large number of pathogens with food plays an important role. Once in the intestine, Salmonella attach to its epithelium and begin to multiply --- into the submucosal space and lymphatic formations of the intestinal wall (then they multiply and die there, releasing endotoxin), because. endotoxin also enters from the outside, then its massive accumulation occurs --- intoxication (severe, with fever, disorders of the NS and CCC) and diarrhea. If a small amount of Salmonella has got into the body with food, then the infection proceeds like gastroenteritis with diarrhea, but without intoxication and fever.

List the test materials and stages of bacteriological diagnosis of foodborne Salmonella infection. Materials are feces, urine, vomit, gastric lavage, food products.

At the preliminary stage, inoculation of the material on a selenite medium or Muller's medium - the growth of the culture will cause the medium to become cloudy. At the 1st stage, sowing on a plate with Endo, Ploskirev, bismult-sulfite agar media (translucent colorless lac-colonies grew on Endo and Ploskirev media, brown and black colonies on bismult-sulfite agar). At the 2nd stage - reseeding of suspicious colonies by injection and stroke on the combined Kligler or Ressel media. On the Kligler medium, the column is yellow, the oblique part is pink, blackening in the lower part of the medium, gas bubbles in the medium. On the Russell medium - a blue column, the beveled part is pink, gas bubbles. At the 3rd stage - identification: a) morphological properties - in a Gram-stained smear (microscopically, gram-negative rods with rounded ends are visible); b) b / chemical sv-va - in the test system API-20E; c) serological identification. At the 4th stage, the results obtained are taken into account and the conclusion is made.

Muller Wednesday- enrichment environment. Compound: MPB, chalk, Lugol's solution, sodium thiosulfate. Purpose: for the accumulation of salmonella when sowing material with a small number of the pathogen and / or heavily contaminated with associated flora. P/d: sodium tetrathionate, which is formed in the medium, inhibits the growth of associated flora --- promotes the growth of salmonella; the environment is cloudy.

selenite environment- enrichment environment. Compound: peptone water, lactose, phosphate buffer, sodium hydroselenite. Purpose: for the accumulation of salmonella and shigella. P/d: sodium hydroselenite inhibits the growth of associated flora --- promotes the growth of salmonella and shigella; the environment is cloudy.

Bismuth Sulfite Agar- selective environment. Compound: MPA, bismuth sulfite, iron sulfate, sodium phosphate, brilliant green. Purpose: for sieving the test material in order to obtain isolated colonies of Salmonella and Proteus. P/d: salmonella forms hydrogen sulfide from iron sulfate, which interacts with colorless bismuth sulfite and converts it into black bismuth sulvit (therefore, Salmonella colonies are black). Growth of gr+ flora and many enterobacteria is inhibited.

Wednesday Guiss- differential diagnostic. Compound: MPA (0.4%), carbohydrates, indicator: bromocresol purple. Purpose: determination of the spectrum of saccharolytic activity in order to identify the isolated culture of enterobacteria, as well as other bacteria. P/d: during the fermentation of carbohydrates to acidic products, the color of the medium turns into yellow, during gas formation - bubbles.

Serological identification of isolated culture of Salmonella.

Serological identification sequence:

1) cultures are tested in the agglutination district with adsorbed polyvalent O-serum containing antibodies against antigen receptors 2, 4, 7, 9, 3, 10.

2) With a positive result, they put the agglutination dose separately with each monoreceptor O-serum, which was part of the polyvalent: receptor 2 (serogroup A), receptor 4 (serogroup B), receptor 7 (serogroup C), etc. .

3) After the serogroup to which the culture belongs is determined, its species affiliation (serovariant) is determined using the agglutination p-tion with adsorbed monoreceptor H-sera against the H-antigens of the first phase of Salmonella that are part of this serogroup. Further, the district of agglutination with adsorbed H-sera against H-antigens of the 2nd phase and finally establish the antigenic formula of the test culture.

Generalized salmonellosis. The most frequently isolated pathogen is hospital strains of S. typhimurium, which have adapted to human org. Occurs in a hospital as nosocomial infection. The source is not only food products, but also bacteria carriers. Contact-household way of distribution. This is an acute intestinal infection with a long severe course of the type of typhoid or toxicoseptic infection. They have all the pathogenicity factors characteristic of S. typhimurium, and are also characterized by increased virulence, long-term preservation on environmental objects, and are resistant to many a/b.

Wednesday Rappoport is an enrichment environment and a differential diagnostic environment. In her compound includes: bile broth 10%, 2% glucose, indicator (sour fuchsin, discolored with alkali; colorless in an alkaline medium, red in an acidic medium). Purpose: for the accumulation of typhoid-paratyphoid bacteria during blood culture of the patient, as well as for the approximate differentiation of typhoid-paratyphoid bacteria. Principle d-i: with the growth of typhoid-paratyphoid bacteria due to the fermentation of glucose, diffuse turbidity and reddening of the medium occur. For paratyphoid bacteria, in contrast to typhoid bacteria, the presence of gas in the float.

Wednesday Endo - differential diagnostic. Compound: MPA, lactose, indicator - basic fuchsin, decolorized with sodium sulfite. Purpose: for sieving the test material in order to obtain isolated colonies. Principle d-I based on the ability of some microorganisms to decompose or not decompose lactose (Salmonella does not decompose and form colorless colonies).

Wednesday Levin is weakly selective and differential diagnostic. In her composition MPA, tactose, indicators: eosin and methylene blue, potassium hydrogen phosphate. Appointment and principle of d-I, see the Endo environment.

Ressel Wednesday Compound: MPA, lactose 1%, glucose 0.1%, indicator (water blue and pink to-ta; in an alkaline environment, pink, in an acidic environment, blue). Purpose: for elimination of "suspicious" colonies in order to isolate pure cultures and determine their enzymatic activity in relation to glucose and lactose. Principle d-i:

Wednesday Kligler - combined, differential diagnostic. Compound: MPA, lactose 1%, glucose 0.1%, sodium thiosulfate, iron II sulfate, indicator - phenol red (in an alkaline medium - red, in an acidic medium - yellow). Purpose: for elimination of "suspicious" colonies in order to identify pure cultures and determine their enzymatic activity in relation to glucose, lactose and the formation of H2S. Principle d-i: enterobacteria, which have the enzyme thiosulfate reductase, reduce thiosulfate to sulfite, while hydrogen sulfide is released, which reacts with iron sulfate - as a result, black iron sulfide is formed.

Serological identification of isolated pure culture using adsorbed monoreceptor O- and H-agglutinating salmonella sera. Serological identification is carried out in the agglutination reaction on a stack with agglutinating adsorbed Salmonella O- and H-sera, these sera can be. 2 types: monoreceptor and polyvalent, they are obtained from specific immune sera by the method of adsorption of antibodies according to Castellani.

Serological identification proceeds sequentially in 3 stages:

1) Establishing the belonging of a culture to serogroups A, B, C, D, E, of the genus Salmonella. In this case, polyvalent O-serum containing antibodies against antigenic receptors 2, 3, 4, 7, 9, 10 is used. Monoreceptor O-sera are used against rare serogroups. When diagnosing paratyphoid A and B, typhoid fever, you can use a mixture of O-sera containing antibodies to receptors 2, 4, 9.

2) With a positive result, to determine whether the test culture belongs to a certain serogroup, the agglutination test is set separately with each monoreceptor O-serum that was part of the polyvalent one: receptor 2 belongs to serogroup A, receptor 4 to serogroup B, receptor 7 - to serogroup C, etc.

3) After determining the serogroup, we determine its species affiliation using the agglutination p-tion with adsorbed monoreceptor H-sera against the H-antigens of the 1st phase of Salmonella, which are part of this serogroup. Then, agglutination is carried out with adsorbed H-sera against H-antigens of the 2nd phase and the antigenic formula of the test culture is finally established.

Bacteriocarrier in typhoid fever. What serological reactions can confirm chronic bacteriocarrier? diagnostics used for this purpose. The only evidence of bacteriocarrier is the isolation from the carrier of cultures S. typhi, S. paratyphi A, S. paratyphi B, using auxiliary methods: serological test and allergic skin test with Vi-typhine (it contains Vi-antigen, which, when interacting with Vi-antibodies gives a local allergic reaction in the form of redness and swelling within 20-30 minutes). A positive p-tion with Vi-typhine indicates that there are Vi-antibodies in the org-me and possibly S. typhi.

Chr. bacteriocarrier is confirmed by RNGA. In this case, Vi-diagnosticum is used, because in the formation of typhoid bacteriocarrier, the manifestation of Vi-antibodies is characteristic; diagnostic titer of the district 1:40.

Methods of infection with typhoid fever. source of infection. Infection with typhoid fever occurs only by the oral route. The source of infection is sick people and bacteria carriers (they are dangerous to others for several years after the illness).

Rapoport Wednesday Rapoport Wednesday

enrichment medium used to isolate Salmonella from blood. For its manufacture, 2% glucose (previously diluted in 5 ml of distilled water) and 1% Andrade indicator (or 0.1% bromine-cresol purple) are added to 10% bile broth with a pH of 7.2. The mixture is poured in 50 ml bottles with floats measuring 60x8 mm. Sterilized for 3 days for 30 minutes with flowing steam. Sow 5-10 ml of fresh blood. Incubated at 37°C for several days. With the growth of Salmonella, the medium becomes cloudy, turns red or yellow, and gas may appear in the float. Transplanted on the environment of Endo, Russell, Olkenitsky.

(Source: Glossary of Microbiology Terms)

See what "Rapoport Wednesday" is in other dictionaries:

- (M. A. Rapoport) solid selective differential diagnostic nutrient medium for the isolation of enterobacteria, containing glycerin, ox bile and sour fuchsin ... Big Medical Dictionary

- (M. A. Rapoport; A. Weintraub) liquid selective nutrient medium for the isolation of typhoid pathogens from the blood, containing meat-peptone broth, bile, Andrade indicator, glucose or mannitol ... Big Medical Dictionary

- (s) (s) an artificial substrate, which is a balanced mixture of nutrients in concentrations and combinations necessary for the growth and division of microorganisms or cells of higher organisms. Adams culture medium, see Adams ... ... Medical Encyclopedia

See Rapoport Wednesday... Big Medical Dictionary

See Rapoport Weintraub Wednesday... Big Medical Dictionary

- (M.A. Rapoport; A. Weintraub) liquid selective nutrient medium for the isolation of typhoid pathogens from the blood, containing meat-peptone broth, bile, Aidrade indicator, glucose or mannitol ... Medical Encyclopedia

- (M.A. Rapoport) solid selective differential diagnostic nutrient medium for the isolation of enterobacteria, containing glycerin, ox bile and acid fuchsinone ... Medical Encyclopedia

- (systems theory) scientific and methodological concept of the study of objects that are systems. It is closely related to the systematic approach and is a specification of its principles and methods. The first version of the general systems theory was ... ... Wikipedia

PARATYPHOID- PARATIF. Contents: Bacteriology ♦ .................. 719 Epidemiology .................. 727 Etiology .............. .............. 728 Statistics. ................... 729 Pathogenesis ..................... 732 Pathological anatomy ..... .......… … Big Medical Encyclopedia

Benzion (Boris) Zakharovich Milner Benzion Milner Date of birth: November 10, 1929 (1929 11 10) (83 years old) Place of birth: Cherkasy, Ukrainian SSR, USSR ... Wikipedia

Rapoport Wednesday

enrichment medium used to isolate Salmonella from blood. For its manufacture, 2% glucose (previously diluted in 5 ml of distilled water) and 1% Andrade indicator (or 0.1% bromine-cresol purple) are added to 10% bile broth with a pH of 7.2. The mixture is poured in 50 ml bottles with floats measuring 60x8 mm. Sterilized for 3 days for 30 minutes with flowing steam. Sow 5-10 ml of fresh blood. Incubated at 37°C for several days. With the growth of Salmonella, the medium becomes cloudy, turns red or yellow, and gas may appear in the float. Transplanted on the environment of Endo, Russell, Olkenitsky.

(Source: Glossary of Microbiology Terms)

• - medium - Nutrient substance used for laboratory cultivation of organisms; many standard biological nutrient C. ...

  Molecular biology and genetics. Dictionary

• - See "Moscow Literary Environment" ...

  Moscow (encyclopedia)

• - 1) the world around; 2) all bodies and phenomena with which the organism is in direct or indirect relationship, the so-called habitat, living environment, ecological environment ...

  The Beginnings of Modern Natural Science

• - solid selective differential diagnostic nutrient medium for the isolation of enterobacteria, containing glycerin, bovine bile and sour fuchsin...

  Big Medical Dictionary

• - liquid selective nutrient medium for the isolation of typhoid pathogens from the blood, containing meat-peptone broth, bile, Andrade indicator, glucose or mannitol ...

  Big Medical Dictionary

• - A term that usually refers to the external environment of an organism, although it can also be used to describe our internal ...

  Great Psychological Encyclopedia

• - Rapoport Anatole - American philosopher, representative of operationalism, biologist, psychologist. In 1941 he graduated from the University of Chicago. Since 1955 ...

  Psychological Dictionary

• - - social space surrounding a person; the zone of the individual's immediate activity, his immediate development and action...

  Pedagogical terminological dictionary

• - middle...

  Brief Church Slavonic Dictionary

• - environment - living conditions and metabolism and energy of organisms, their aggregates with the outside world ...

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• - Iosif Abramovich, geneticist, corresponding member of the USSR Academy of Sciences, Hero of Socialist Labor. In 1935-48 he worked at the Institute of Experimental Biology of the USSR Academy of Sciences...

  Russian encyclopedia

• - : See also: - grinding medium - ambient medium - saturating medium - corrosive medium - protective medium - hardening medium - neutral medium - working medium - diathermic medium...

  Encyclopedic Dictionary of Metallurgy

• - 1) the world around; a concept introduced into philosophy and sociology by Taine. The theory of the environment recognized a person as deeply dependent on the world around him and therefore morally completely irresponsible ...

  Philosophical Encyclopedia

• - 1. the environment, conditions, the totality of people associated with these conditions 2. the substance that fills the space 3. the environment, the totality of natural conditions ...

  Big Economic Dictionary

• - Jewish scientist originally from Lviv; was a rabbi in Tarnopol and Prague ...

  Encyclopedic Dictionary of Brockhaus and Euphron

• - Russian geneticist, corresponding member of the USSR Academy of Sciences, Hero of Socialist Labor ...

  Big encyclopedic dictionary

"Rapoport Wednesday" in books

Wednesday

Wednesday Two letters from you arrived at lunchtime today; they would not be read, but laid out on the table, hiding their face in them and losing their minds. But then it turns out how good it is that you have almost lost it, because then you cling to the rest as long as possible. And that's why my 38

Wednesday

Wednesday It is difficult to tell the truth, because although she is alone, she is alive, and therefore she, like all living things, has a changeable face (“krasna vubec nikdy, vazne ne, snad nekdy hezka”). If I answered you on the night from Monday to Tuesday, it would be terrible, I lay in bed as if under torture, I answered all night

Wednesday

Wednesday You write: “Ano mas pravdu, mam ho rada. Ale F., i Tebe mam rada. I read this phrase very carefully, word by word, especially I linger on “and”, that’s right, you wouldn’t be Milena if it weren’t true (and what would I be if it weren’t for you?), - and even better that you are writing this in Vienna than if

Wednesday

Wednesday After all, courage yields results. First of all: Gross is probably right in many respects, if I understand him correctly; at least the fact that I am still alive speaks in favor of his theory - and in fact, if you take into account my internal balance of forces, I have long been no longer a tenant on this

RAPOPORT VLADIMIR

RAPOPORT VLADIMIR RAPOPORT VLADIMIR (cameraman: “Girlfriends” (1936, with A. Shafran), “On the Border” (1938), “Frontline Friends” (1941), “She Defends the Motherland” (1943), “Sons”, “Marine battalion "(both - 1946)," Young Guard "(1948)," Star "(1949, 1953, with S. Ivanov)," Quiet Don "(1957-1958)," People and

Good Vladimir Rapoport